Saturday Night Live aired its thirty-fifth season during the 2009–2010 television season on NBC. This season began on September 26, 2009 and ended on May 15, 2010.

A total of 22 episodes were broadcast during the show's eight-month-long season, which included a two-week break in February due to the 2010 Winter Olympics. The season was accompanied by three prime-time episodes of Saturday Night Live Weekend Update Thursday and three prime-time SNL clip shows.

Prior to the start of the season, many cast changes occurred. Darrell Hammond, the last cast member from the 1990s, left the show after a record 14 seasons in the cast. Featured players Michaela Watkins and Casey Wilson were both let go from the show before the start of the season.

To fill their absence the show brought in two new featured players, Nasim Pedrad and Jenny Slate. Abby Elliott and Bobby Moynihan continued as featured players.

A notable moment of the season was when an internet campaign was created to get actress Betty White to host an episode of the show. The campaign was started in early 2010 on Facebook and the group was called "Betty White to Host SNL!" The campaign was successful, and White became the oldest person ever to host the show. For White's episode, Lorne Michaels brought back former cast members Rachel Dratch, Tina Fey, Ana Gasteyer, Amy Poehler, Maya Rudolph and Molly Shannon. The episode garnered its highest ratings in over a year. with a rating of 5.8 in the 18–49 rating, demographic and with 12.1 million viewers overall.

FBI agent Neville Flynn boards a flight from Honolulu, Hawaii to Los Angeles, escorting a key witness to testify against a mob boss at an upcoming trial. An on-board assassin releases a crate full of hundreds of deadly venomous snakes in an attempt to eliminate the witness. Flynn and a host of frightened passengers and crew must band together to survive the slithery threat.