Dick Tracy's foe for this serial is the crime boss and Masked Mystery Villain The Spider/The Lame One and his Spider Ring. In the process of various crimes, including using his Flying wing and sound weapon to destroy the Bay Bridge in San Francisco and stealing an experimental "Speed Plane", the Spider captures Dick Tracy's brother, Gordon. The Spider's minion, Dr. Moloch, performs a brain operation on Gordon Tracy to turn him evil, making him secretly part of the Spider Ring and so turning brother against brother.

When the owner of a stagecoach station is killed, a gunman takes his place.

A spirited dressmaker's small store flourishes into a business empire in the midst of the Texas oil boom of the 1940s.

An ex-convict drifter and his flawed young partner are made sheriff and deputy of a Western town.

A year after a violent train robbery the Pinkerton detective agency hires a bounty hunter to find the three remaining killers. He tracks them to Twin Forks but has no clue to their identity. Tensions surface as just his presence in town acts as a catalyst.

In New York City, a newly hired photographer becomes embroiled in a scandal when her photo is mistaken for evidence of a murder and she must try to prove her own innocence.

Magazine owners sell a revealing photo, then play detective when the deal leads to murder.

An insurance salesman, Albert Tuttle, is hired as a body guard for a millionaire.

A fugitive receives help from a victim's sister as he tries to clear his name of robbery and murder charges.

When two US cavalrymen transporting a gold shipment get killed, US Army Intelligence investigator John Haven goes undercover to a mining and logging town to find the killers.