Dick Tracy's foe for this serial is the crime boss and Masked Mystery Villain The Spider/The Lame One and his Spider Ring. In the process of various crimes, including using his Flying wing and sound weapon to destroy the Bay Bridge in San Francisco and stealing an experimental "Speed Plane", the Spider captures Dick Tracy's brother, Gordon. The Spider's minion, Dr. Moloch, performs a brain operation on Gordon Tracy to turn him evil, making him secretly part of the Spider Ring and so turning brother against brother.

An American Army tank unit is trapped in a small French town in the Ardennes unaware that a spy amongst them is providing the German counterattack with vital battle information.

A nightclub singer uses alcohol in excess to sooth her painful life.

An oil engineer surrounded by foul play helps an heiress bring in a well.

A terrified stewardess is stalked by her psychotic estranged husband.

On parole after three years in prison, a football player encounters the man who framed him.

Various performers and backstage crew come under suspicion when a dancer is found murdered at a burlesque theatre.

Joseph Cotten plays an assistant bank manager who steals $1,000,000 from the safe late on a Friday and then plans to flee to Brazil over the weekend.

Respected black cavalry Sergeant Brax Rutledge stands court-martial for raping and killing a white woman and murdering her father, his superior officer.