Dick Tracy's foe for this serial is the crime boss and Masked Mystery Villain The Spider/The Lame One and his Spider Ring. In the process of various crimes, including using his Flying wing and sound weapon to destroy the Bay Bridge in San Francisco and stealing an experimental "Speed Plane", the Spider captures Dick Tracy's brother, Gordon. The Spider's minion, Dr. Moloch, performs a brain operation on Gordon Tracy to turn him evil, making him secretly part of the Spider Ring and so turning brother against brother.

Wanted outlaws have mysteriously disappeared. Ranger Captain Henley and Steve have a plan to find them. Steve becomes a wanted man by faking the killing of Henley. Not only is he now in trouble as both the Rangers and the Mexican Rurales are after him, but Smiley knows him and may expose his masquerade to the bad guys.

Gene Autry and his sidekick Frog look into a phony oil scam being perpetrated on a mission orphanage.

In his starring debut, Roy gets elected to Congress in order to bring water to the ranchers in his district. In Washington, he learns he needs the backing of a key congressman and gets that man to go west for an inspection trip. When the congressman is initially unimpressed, Roy gets the inspection party stranded without water to show the true conditions.